Compliance Methods / Official Methods/ Provisional Methods
Cel-A ELAP#2647
Microbiology of Drinking Water        
Method Analyte Technique/
Medium
Sample Size/Container Preservative Holding Times
SM9215B Heterotrophic Bacteria Pour plate 100 mL/ Sterile Plastic 4⁰-6⁰ C; sodium thiosulfate 30 hrs
SimPlate Heterotrophic Bacteria SimPlate 100 mL/ Sterile Plastic 4⁰-6⁰ C; sodium thiosulfate 30 hrs
SM9221A,B Total Coliform MTF1 100 mL/ Sterile Plastic 4⁰-6⁰ C; sodium thiosulfate 30 hrs
SM9221E (MTF/EC) Fecal Coliform  MTF 100 mL/ Sterile Plastic 4⁰-6⁰ C; sodium thiosulfate 30 hrs
SM9223 Total Coliform Colilert 2,3 100 mL/ Sterile Plastic 4⁰-6⁰ C; sodium thiosulfate 30 hrs
SM9223 E. coli Colilert 100 mL/ Sterile Plastic 4⁰-6⁰ C; sodium thiosulfate 30 hrs
EPA 600/J-99/225 (MI) Total Coliform MF4 100 mL/ Sterile Plastic 4⁰-6⁰ C; sodium thiosulfate 30 hrs
EPA 600/J-99/225 (MI) E. coli MF 100 mL/ Sterile Plastic 4⁰-6⁰ C; sodium thiosulfate 30 hrs
Microbiology of Drinking Water Source (complies with EPA source water treatment rules)  
Method Analyte Technique/
Medium
Sample Size/Container Preservative Holding Times
SM9221A,B,C Total Coliform MTF/ LTB 100 mL/ Sterile Plastic 4⁰-6⁰ C; sodium thiosulfate 30 hrs
SM9221E Fecal Coliform  MTF/ A-1 100 mL/ Sterile Plastic 4⁰-6⁰ C; sodium thiosulfate 30 hrs
SM9223 Total Coliform Colilert 100 mL/ Sterile Plastic 4⁰-6⁰ C; sodium thiosulfate 30 hrs
EPA 600/J-99/225 Total Coliform MF- MI agar 100 mL/ Sterile Plastic 4⁰-6⁰ C; sodium thiosulfate 30 hrs
SM9223B E. coli Colilert 100 mL/ Sterile Plastic 4⁰-6⁰ C; sodium thiosulfate 30 hrs
SM9221B.1/SM9221F E. coli MTF/ EC+MUG 100 mL/ Sterile Plastic 4⁰-6⁰ C; sodium thiosulfate 30 hrs
EPA 1603 E. coli MF/ mTEC 100 mL/ Sterile Plastic 4⁰-6⁰ C; sodium thiosulfate 30 hrs
EPA 1604 E. coli MF 100 mL/ Sterile Plastic 4⁰-6⁰ C; sodium thiosulfate 30 hrs
EPA 1602 Somatic/Male specific Phage Plaque Assay 1000 ml/ Sterile Plastic 4⁰-6⁰ C; sodium thiosulfate 30 hrs
SM 9228 Aerobic Endospores MF 1000 ml/ Sterile Plastic 4⁰-6⁰ C  30 hrs
EPA 1623/1623.1 Cryptosporidium/Giardia Filtration / IMS / FA5 10 L/ cubitainer or filter (contact lab) 4⁰-6⁰ C 96 hrs
EPA 1615 (UCMR3)6 Enteric Viruses7  Cell Culture or RTqPCR8 / qPCR9 Nanoceram filter (contact lab) 4⁰-6⁰ C 72 hrs
Total mycrocystins (UCMR4)6 Cyanotoxins ADDA ELISA Assay 250 ml/ glass or PETG 4⁰-6⁰ C; sodium thiosulfate 5 days
Microbiology of Recreational Water (including Swimming Pools)     
Method Analyte Technique/
Medium
Sample Size/Container Preservative Holding Times
SM9221A,B,C Total Coliform MTF/ LTB 100 mL/ Sterile Plastic 4⁰-6⁰ C 6 hrs
SM9221E Fecal Coliform  MTF/ EC 100 mL/ Sterile Plastic 4⁰-6⁰ C 6 hrs
SM9223 Total Coliform and E. coli Colilert 100 mL/ Sterile Plastic 4⁰-6⁰ C 6 hrs
EPA 1600 Enterococci  MF/ mEI 100 mL/ Sterile Plastic 4⁰-6⁰ C 6 hrs
IDEXX Enterococci  Enterolert10 100 mL/ Sterile Plastic 4⁰-6⁰ C 6 hrs
EPA 1611 Enterococci  qPCR 200 mL/ Sterile Plastic 4⁰-6⁰ C 6 hrs
SM9260J (CDC ELITE) Legionella Culture method 1L/ 250 mL/ Sterile Plastic 4⁰-6⁰ C 6 hrs
Microbial Source Tracking (MST) in Surface Water      
Method Analyte Technique/
Medium
Sample Size/Container Preservative Holding Times
EPA Method B Total Bacteroidales MF / qPCR 200 mL/ Sterile Plastic 4⁰-6⁰ C 6 hrs12
EPA Method B/ CA MSIM11 Human  Marker MF / qPCR 200 mL/ Sterile Plastic 4⁰-6⁰ C 6 hrs12
EPA Method B/ CA MSIM Canine  Marker MF / qPCR 200 mL/ Sterile Plastic 4⁰-6⁰ C 6 hrs12
EPA Method B/ CA MSIM Ruminant Marker MF / qPCR 200 mL/ Sterile Plastic 4⁰-6⁰ C 6 hrs12
EPA Method B/ CA MSIM Seagull Marker MF / qPCR 200 mL/ Sterile Plastic 4⁰-6⁰ C 6 hrs12
EPA Method B/ CA MSIM Horse  Marker MF / qPCR 200 mL/ Sterile Plastic 4⁰-6⁰ C 6 hrs12
Other species specific markers are available; please contact the lab for information
1 MTF= Multiple Tube Fermentation
2 Presence-Absence assay using Idexx Coliert® 24 or 18
3 Quantitray assay using Idexx Coliert® 24 or 18
4 MF= Membrane Filtration
5 IMS= immunomagnetic separation; FA = Flourescence Assay
6 UCMR = Unregulated Contaminant Monitoring Rule
7 Enteric viruses include Enterovirus, Norovirus, Adenovirus, and Rotavirus
8 RT-qPCR= Reverse Transciptase-quantitiative Polymerase Chain Reaction
9 qPCR= quantitiative Polymerase Chain Reaction
10 Quantitray assay using Idexx Enterolert® 
11The California Microbial Source Identification Manual
12Unless filtered on site
Microbiology of Biosolids Under the EPA Part 503 Biosolids Rule (40 CFR Part 503)   
Method Analyte Technique/
Medium
Sample Size/Container Preservative Holding Times
SM9221E Fecal Coliform MTF/ A-1 or EC 100 g or 100 mL in Sterile Container 4⁰-6⁰ C 24 hrs
SM9260B Salmonella Species Enrichment and serotyping 100 g or 100 mL in Sterile Container 4⁰-6⁰ C 24 hrs
ASTM Method D 4994-89 Enteric Viruses Flocculation/ Cell Culture 1-4 L in Sterile Container 4⁰-6⁰ C 24 hrs
EPA 1615 Enteric Viruses Cell Culture or RTqPCR / qPCR Contact lab for details Freeze (store at 0⁰ C) 2 weeks
EPA 1602 Male-specific and/or Somatic bacteriophage Plaque Assay Contact lab for details 4⁰-6⁰ C 24 hrs
EPA 1680 Fecal Coliforms in Sewage Sludge MTF/ LTB 100 g or 100 mL in Sterile Container 4⁰-6⁰ C 24 hrs
EPA 1681 Fecal Coliforms in Sewage Sludge MTF/ A-1 100 g or 100 mL in Sterile Container 4⁰-6⁰ C 24 hrs
Microbiology of Wastewater (Gray Water, Black Water, Rain Water) & Cooling Towers   
Method Analyte Technique/
Medium
Sample Size/Container Preservative Holding Times
SM9215B Heterotrophic Bacteria Pour plate 100 mL/ Sterile Plastic 4⁰-6⁰ C 6-8 hrs
SM9221B Total Coliform MTF/ LTB 100 mL/ Sterile Plastic 4⁰-6⁰ C 6-8 hrs
SM9221C,E Fecal Coliform  MTF/ EC 100 mL/ Sterile Plastic 4⁰-6⁰ C 6-8 hrs
SM9221C,E  Fecal Coliform  MTF/ A-1 100 mL/ Sterile Plastic 4⁰-6⁰ C 6-8 hrs
SM9230C Fecal Streptococci MF/ mE 100 mL/ Sterile Plastic 4⁰-6⁰ C 6-8 hrs
SM9230C Enterococci  MF/ mE 100 mL/ Sterile Plastic 4⁰-6⁰ C 6-8 hrs
Enterolert Enterococci  CF-MUG/ Quantitray 100 mL/ Sterile Plastic 4⁰-6⁰ C 6-8 hrs
EPA 1600 Enterococci  MF/ mEI 100 mL/ Sterile Plastic 4⁰-6⁰ C 6-8 hrs
SM9223 E. coli EC-MUG/ Quantitray 100 mL/ Sterile Plastic 4⁰-6⁰ C 6-8 hrs
SM9260J (CDC ELITE) Legionella Culture method 1 L/  Sterile Plastic 4⁰-6⁰ C 24-48 hrs
EPA 1615 Enteric Viruses Cell Culture or RTqPCR / qPCR 1 liter /Plastic Sterile 4⁰-6⁰ C 72hrs
Microbiology of Shellfish Meat and Growing Waters      
Method Analyte Technique/
Medium
Sample Size/Container Preservative Holding Times
APHA1 Shellfish Meat TC/FC2 MTF 200 grams unopened shells/ Sterile Containers 4⁰-6⁰ C 24 hrs
APHA Shellfish Meat Plate Counts SPC2 200 grams unopened shells/ Sterile Containers 4⁰-6⁰ C 24 hrs
APHA Shellfish Growing       Waters TC/FC MTF 100 mL/ Sterile Plastic 4⁰-6⁰ C 6-8 hrs
AOAC3 Shellfish Growing       Waters FC A-1 100 mL/ Sterile Plastic 4⁰-6⁰ C 6-8 hrs
WPCF4/AOAC Shellfish Growing       Waters FC MTF LTB/EC & A-1 100 mL/ Sterile Plastic 4⁰-6⁰ C 6-8 hrs
Microbiology of Food (FDA Recommended Methods)      
Example Food Product Analyte Technique/
Medium
Sample Size/Container * Preservative Holding Times
Consumer packaged good; produce Salmonella Immunochromatographic test/serotoyping 100 g or 100 ml liquid 4⁰-6⁰ C A short as possible
Consumer packaged good; produce B.cereus Enrichment and biochemical tests 100 g or 100 ml liquid 4⁰-6⁰ C; note; dehydrated food is stored at room temperature 2 weeks if frozen
Consumer packaged good; produce E.coli O157:H7 Immunochromatographic test/serotoyping 100 g or 100 ml liquid 4⁰-6⁰ C; note; dehydrated food is stored at room temperature A short as possible
Juice, Cider, Milk, or Bottled Water Parasites (Cyclospora/ Cryptosporidium) Filtration/ elution and qPCR screening viariable; 10 mL to 10 liter 4⁰-6⁰ C A short as possible
Infant formula Cronobacter (Enterobacter) qPCR 100 g or 100 ml liquid stored at room temperature/ 4⁰-6⁰ C; for liquid A short as possible
Shellfish, produce Norovirus qPCR Contact lab 4⁰-6⁰ C 2 weeks if frozen
* Please note that sample size is variable for food matricies
1APHA – American Public Health Association, Recommended Procedures for the Examination  of Sea Water and Shellfish, 4th ed, 1970
2TC/FC = Total Coliform / Fecal Coliform
3SPC = Standard Plate Count
4AOAC – Association of Official Analytical Chemists – Official Methods of Analysis, 15th ed
5WPCF – Journal of Water Pollution Control Federation, 59, 8 (1987)

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